Klinik für Neurologie

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Methods and Techniques

Animal models:

 mice (wildtype and transgenic), postnatal and adult  rats, postnatal and adult 

Experimental Lesion Models:

 retinal ischemia (elevated IOP)glaucoma (episcleral vein cauterization)optic nerve axotomyoptic nerve crush

 

Intraocular delivery of substances:

defined factors e.g., EPO
substances, e.g. statins

Cell culture:

 Primary culture:     retinal ganglion cells
                            Müller glia
                            microglia
  Cell lines:              RGC-5 (Retinal ganglion cells)
                            MIO-M1 (Müller glia)

Cell isolation:

 FACS
immunopanning

Molecular biology:


 qPCR

Protein biochemistry:

Western blot
ELISA

Immunocyto- and histochemistry:

multi-colour immunofluorescence
in vivo tracing of visual projection pathways

Microscopy:

confocal laser scanning microscopy (LSM) with multi-channel analysis and 3-D reconstruction

In vivo analysis of visual function:

virtual optomotor systemanalysis of intrinsic optical signals (IOS)
Behavioural tests:

 visual water task

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